Biofuel- Science Method

Plan Aim: The aim of this investigate is to identify the optimal pH for which the enzyme, amylase, whole shebang best at. Hypothesis: I predict that enzyme bodily process will decrease if it steers away from 6-8 pH as this is the optimum pH start out for which the enzyme amylase works best. Variables: Dependant Variables: The time it takes the enzyme to react. maintain Variables: The summate of starch doing The bar of enzyme solution The amount of Iodine solution The amount of water in ML The time taken for the reaction. strong-minded Variables: The range of pH, between 6-8 pH. Method: 1. Firstly, inject the buffer verbal contraceptives into a test-tube containing 20 ml of water (at room temperature). 2. at a time the buffer tablet has dissolved into the water, add 10 ml of starch solution. 3. later the starch solution has been added, add 10 ml of enzyme so lution to the current solution, simultaneously start the timekeeper and record at 1 minute intervals. 4. In addition, get into iodine in the dropping tile in lurch to identify if starch is currently present. 5. Repeat each experiment 3 times. Equipment: 1. drop Tile 2. Pipette 3. Test subway system 4. Starch ancestor 5. Iodine Solution 6. Enzyme Solution 7. Beaker(s) 8. water supply 9. Measuring Cylinder (ml) 10. oecumenical Indicator 11.

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